Antibacterial activity test of n-hexane, ethyl acetate, and water fractions of the flat-top mille graines (Hedyotis corymbosa L) herb against the growth of the propionibacterium acnes bacteria
DOI:
https://doi.org/10.33292/kpqgdk71Keywords:
Lat-top Mille Graines, Fractions, PropionibacteriumAbstract
Acne, a chronic obstructive and inflammatory skin condition affecting pilosebaceous units, is often linked to Propionibacterium acnes, a bacterium that disrupts the stratum corneum and stratum germinativum by secreting sebum, leading to damage in the walls of skin pores. This study aims to identify the chemical compounds present in the n-hexane, ethyl acetate, and water fractions of flat-top mille graines or diamond flower, locally known as rumput mutiara (Hedyotis corymbosa L.) herbal extract. Additionally, it seeks to determine the optimal concentration and the fraction exhibiting the most potent antibacterial activity against Propionibacterium acnes, with the aim of inhibiting bacterial growth associated with acne. This study employed laboratory experimental methods. Extraction was conducted through the maceration method using ethanol solvent. Fractionation was achieved through liquid-liquid extraction using n-hexane, ethyl acetate, and water solvents. Phytochemical screening and inhibition tests were performed using Clindamycin as the positive control and DMSO as the negative control. Data analysis involved the One-Way ANOVA method followed by the LSD test for further analysis. Phytochemical screening of the flat-top mille graines (Hedyotis corymbosa L.) fractions revealed positive results for various compounds. The n-hexane fraction contained flavonoid and triterpenoid compounds, the ethyl acetate fraction exhibited tannin, saponin, flavonoid, and steroid compounds, while the water fraction contained alkaloids, tannins, saponins, flavonoids, and steroids. In terms of antibacterial activity against Propionibacterium acnes, the n-hexane fraction at a concentration of 15% displayed a medium inhibition zone diameter of 8.6 mm, whereas the ethyl acetate and water fractions both exhibited strong inhibition zones with diameters of 16.6 mm at the same concentration. The optimal concentration for inhibiting Propionibacterium acnes was found to be 15% for both the ethyl acetate and water fractions. Formulation of the herbal fractions of flat-top mille graines (Hedyotis corymbosa L.) is recommended based on these findings.
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